Applications and Approaches for Three-Dimensional Precision-Cut Lung Slices. Disease Modeling and Drug Discovery
نویسندگان
چکیده
منابع مشابه
Biaxial distension of precision-cut lung slices.
The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
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The precision-cut lung slice (PCLS) technique is widely used to examine airway responses in different species. We developed a method to study nerve-dependent bronchoconstriction by the application of electric field stimulation (EFS) to PCLS. PCLS prepared from Wistar rats were placed between two platinum electrodes to apply serial rectangular impulses (5-100 Hz), and bronchoconstriction was stu...
متن کاملReproducible uniform equibiaxial stretch of precision-cut lung slices.
Simulating ventilator-induced lung injury (VILI) in the laboratory requires stretching of lung alveolar tissue. Whereas precision-cut lung slices (PCLSs) are widely used for studying paracrine signaling pathways in the lungs, their use in stretch studies is very limited because of the technical challenge of fixing them to a stretchable substrate, stretching them uniformly, or holding them in a ...
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Precision-cut lung slices (PCLS) are an established ex vivo alternative to in vivo experiments in pharmacotoxicology. The aim of this study was to evaluate the potential of PCLS as a tool in nanotoxicology studies. Silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticles as well as quartz particles were used because these materials have been previously shown in several in vitro and in vivo studie...
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Rosiglitazone elicits in vitro relaxation in airways and precision cut lung slices from a 1 mouse model of chronic allergic airways disease 2 3 1, Chantal Donovan, Simon R Bailey, Jenny Tran, Gertruud Haitsma, Zaridatul A 4 Ibrahim, Simon R Foster, Mimi L K Tang, 1,2,4+ Simon G Royce, Jane E Bourke 5 6 Biomedicine Discovery Institute and Department of Pharmacology, Monash University, 7 Clayton,...
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ژورنال
عنوان ژورنال: American Journal of Respiratory Cell and Molecular Biology
سال: 2020
ISSN: 1044-1549,1535-4989
DOI: 10.1165/rcmb.2019-0276tr